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Shanghai RealGene Bio-Tech, Inc.

 
 
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Protein A Resin 

  Category : Biotech->Biotech Processing->analytical products

     
Protein A Resin
       

  Product Specification
 
  Model No:      RGM00089
  Country  CN
  Brand  realgene
  Materials  glutathione,slurry
  Size  1 mg in 1 ml of storage buffer    
  Color  white
  Packing  1 piece/bag
  Minimum Order  1~5
  Payment Term  100% T/T advance

 

    Product Detail
  HRP-Protein A
Cat. No.: RGM00089
Price:US$194.00/Piece

Size: 1 mg in 1 ml of storage buffer

Molar Ratio of HRP/Protein A: 2:1.
Concentration: 1 mg /ml in PBS (pH 7.4) containing 10 mg /ml BSA and 0.01% Thimerosal.

Description:Staphylococcal Protein A binds strongly and specifically to the Fc region of immunoglobulin molecules, especially IgG of many species such as Human, Rabbit, Pig, etc. .The product contains 5 high affinity binding sites that are capable of interacting with the Fc region of IgG. HRP-Protein A is produced by covalently linking HRP to Protein A, which can be used in immunodetection such as immunoblotting (western or dot blot), ELISA, and immunohistochemistry.

Storage: Store at 4 centi-degree for short term (one-month ).Aliquot and store at -20 centi-degree for longer storage. Avoid repeated freeze/thaw cycles.If slight precipitation occurs upon prolonged storage ,clarify the solution by centrifugation before use.

Working Dilution : Working concentrations for specific applications should be detemined by titration assay. Appropriate concentrations will be affected by several factors including primary antibody, antigen amount,sensitivity of detection method,temperature,and incubation time,etc.The following dilution ranges are just a guideline:

Dot blot: 1:5,000~1:20,000
Western blot: 1:5,000~1:20,000
ELISA: 1:50,000~1:250,000
Immunohistochemistry: 1:500~ 1:2,5 00

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